Herpes Simplex Virus II Diagnostic Kit (PCR-Fluorescence Probing)

Herpes Simplex Virus II (HSV II) is commonly found as an asymptomatic genital infection, which spreads via direct contact with the infected site. Serological tests currently available are neither highly qualitative nor high throughput. The HSV II Fluorescent PCR diagnostic test kit is designed for in vitro quantification of HSV II using the composite probe technique which provides a rapid auxiliary diagnosis of this pathogen. Detailed clinical study has validated the high specificity, sensitivity, and reproducibility of the kit with analytical sensitivity of 1000 copies/mL in the sample. The kit can be used for early diagnosis of clinical infection of HSV II. The kit contains reagents for nucleic acid extraction, realtime-PCR detection and quality control.

Catalogue #: ABI - Herpes Simplex Virus II PCR Diagnostic Kit

Principles of the test

The kit has specifically designed primers as well as a composite fluorescent probe thereby allowing utilization of real-time fluorescent PCR. The genetic sequence of HSV II can be targeted through realtime-PCR amplification.

Key Features

The kit contains ready-to-use reagents, offers fast real-time detection and provides excellent sensitivity and high specificity. It is also optimized for use in automated liquid handling workstations. By combining this kit with Aurora’s VERSA™ workstations, laboratories can fully automate the amplification process thereby reducing cost and operator error, while increasing efficiency.

Product Features:

• 48 tests per kit (25µL reaction)
• Rapid detection of HSV II
• All necessary PCR reagents pre-mixed and ready-to-go
• Applicable for most commercially available realtime PCR instruments
• Standard instrument set-up parameters
• Accurate controls to confirm findings
• Colour coded reagent vials

• DNA extraction reagent
• PCR detection reagent
• PCR Buffer
• Primer/Probe/dNTPs
• Taq polymerase
• MgCl2
• Quality control reagent
• HSV II positive control
• HSV II quantitication reference
• Negative control
• Diluent

Dynamic range of kit

Under optimal PCR conditions the kit has very high priming efficiencies of >95% and can detect between 1X108 and 1X103 copies of target template.

Handling & Storage Guidelines

Kit Storage & Shelf life - 12 months at -20ºC±5ºC. Repeated freezing and thawing should be avoided.

Sample Requirements & Capabilities

Genito-urinary tract secretion swab and bleb fluid.

Sample collection, storage and transportation

Please follow the current guidelines and ordinance on HSV II specimen collection, handling, storage, packaging, and transport requirements.

Disclaimer: The kit is for detection of Herpes Simplex Virus II samples, and can be used for the laboratory diagnosis and monitoring the particular virus infection. The result is for research only, and should not be used in clinical diagnosis.

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