VERSA Applications

Solid-Phase Peptide Synthesis using VERSA Spotter Workstation

Peptide synthesis involves linking of multiple amino acids via peptide bonds for the production of peptides. This process mimics the biological process of producing long peptides (proteins). In solid-phase peptide synthesis (SPPS), the peptide is 'immobilized' on a solid surface and can be retained during washing of liquid-phase reagents and synthesis by-products. Solid-phase peptide synthesis also allows the synthesis of natural peptides which are difficult to express in bacteria, the incorporation of unnatural amino acids, and peptide/protein backbone modification. In addition this process permits the synthesis of D-proteins generating extremely high yield in each step and provides a solution problems associated with sequence dependent synthesis.

Aurora’s VERSA Spotter is an automated liquid handling platform, capable of performing automatic pipetting for sub-microliter dispensing and arraying applications in contact printing applications. This system spots Fmoc or t-Boc amino acid residues in dimethylformamide (DMF) on to solid surfaces, such as membranes, slides, filter paper or any other suitable array surface, in user-defined sequences. This workstation enables the reliable synthesis of different peptides in a simple and reproducible manner with extremely high yield and accuracy. The robotic arm is controlled by an external computer, allowing the user to micro-control the desired range of movement, sequence of individual peptides and standardize the spacing between spots.

Features

• Highly customizable deck
• Nano-precision dispensing
• No impact on sensitive surfaces
• User friendly software interface
• Performs desired spotting procedure irregardless of the starting material and substrate used
• Most common spotting techniques can be efficiently automated
• Works on any array surface including slides and membranes

Applications

• Protein/Peptide arrays
• Nucleic acid arrays
• Miniaturized assays
• Microarrays of solutions and reagents

For more information, please review the Peptide Synthesis Application Note or contact us directly to discuss your specific application requirements. Thank you for considering AURORA to solve your lab automation needs.